Full usage
usage: auriclass [-h] [-n NAME] [-o OUTPUT_REPORT_PATH] [--fastq] [--fasta] [--no_qc]
[--log_file_path LOG_FILE_PATH] [--verbose] [--debug] [--version]
[--expected_genome_size EXPECTED_GENOME_SIZE EXPECTED_GENOME_SIZE]
[--non_candida_threshold NON_CANDIDA_THRESHOLD]
[--high_dist_threshold HIGH_DIST_THRESHOLD] [-r REFERENCE_SKETCH_PATH]
[-c CLADE_CONFIG_PATH] [-k KMER_SIZE] [-s SKETCH_SIZE]
[-m MINIMAL_KMER_COVERAGE]
read_file_paths [read_file_paths ...]
AuriClass predicts Candida auris clade from Illumina WGS data
options:
-h, --help show this help message and exit
REQUIRED:
read_file_paths Paths to read files
Main arguments:
-n NAME, --name NAME Name of isolate (default: isolate)
-o OUTPUT_REPORT_PATH, --output_report_path OUTPUT_REPORT_PATH
Path to output report (default: report.tsv)
--fastq Input files are fastq files (default: False)
--fasta Input files are fasta files (default: False)
--no_qc Skip extended QC (default: False)
--log_file_path LOG_FILE_PATH
Path to log file (default: None)
--verbose Verbose output (default: False)
--debug Very verbose output (default: False)
--version show program's version number and exit
QC arguments:
--expected_genome_size EXPECTED_GENOME_SIZE EXPECTED_GENOME_SIZE
Expected genome size range. Defaults 11.4-14.6 Mb are based on 150
NCBI genomes and take mash genome size overestimation into
account. (default: [11400000, 14900000])
--non_candida_threshold NON_CANDIDA_THRESHOLD
If the minimal distance from a reference sample is above this
threshold, the sample might not be a Candida sp. (default: 0.01)
--high_dist_threshold HIGH_DIST_THRESHOLD
If the minimal distance from a reference sample is above this
threshold, a warning is emitted. See the docs for more info.
(default: 0.003)
Other arguments
NOTE: Only change these settings if you are doing something special.
NOTE: This will require rebuilding the reference sketch and recalibration of thresholds!:
-r REFERENCE_SKETCH_PATH, --reference_sketch_path REFERENCE_SKETCH_PATH
Path to reference sketch (default: )
-c CLADE_CONFIG_PATH, --clade_config_path CLADE_CONFIG_PATH
Path to clade config (default: )
-k KMER_SIZE, --kmer_size KMER_SIZE
Kmer size (default: 27)
-s SKETCH_SIZE, --sketch_size SKETCH_SIZE
Sketch size (default: 50000)
-m MINIMAL_KMER_COVERAGE, --minimal_kmer_coverage MINIMAL_KMER_COVERAGE
Minimal kmer coverage (default: 3)